![]() These results suggest that inter-particle interactions, together with the intrinsic chirality of the virus, play an important role in the origin of the helical twist in the cholesteric phase. The resulting particles can only form a nematic phase without any macroscopic helicity. UV-visible spectra of fd viruses labeled with dyes (fd-FITC and fd-ROX) For the UV-visible spectra, diluted solutions of the wild-type fd viruses, dye-labeled viruses and free dyes were prepared in TRIS-HCl NaCl buffer (I 15 mM, pH 8.2). Besides these modifications targeting the amino groups, the surface carboxyl groups were coupled with amino compounds so that the surface charge of fd viruses was reversed from negative to positive. In addition, the chemically modified viruses exhibit a well-defined first order phase transition between the isotropic liquid and the liquid-crystalline state. The degree of unwinding of the helical periodicity (or cholesteric pitch) depends on the structure of the labeled molecules. The resulting chemically modified viruses still form a cholesteric phase in an aqueous suspension, but with a lower helicity compared to suspensions made of wild-type fd viruses. For this purpose, the filamentous fd virus has been subjected to various chemical modifications on its capsid proteins: several organic molecules of different sizes and structures, including two popular fluorescent dyes ( fluorescein and rhodamine), were coupled to the surface amino groups of the virus. In this work, we studied the influence of viral surface properties on the self-assembly of rod-like fd virus suspensions into the chiral nematic phase. ![]() Filamentous viruses of similar molecular and subunitary structure can form either a liquid crystalline nematic phase or a chiral nematic – also called cholesteric – phase, which is an elegant example of how self-organization of nanorods, with otherwise similar properties, can be affected by only subtle differences of surface properties and of the arrangement of the constituent subunits.
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